Fmoc-Phe-Wang Resin (100–200 Mesh): Preloaded Resin for C-Terminal Phenylalanine Peptides
Fmoc-Phe-Wang Resin is a preloaded polystyrene-based solid-phase resin designed for Fmoc/tBu solid-phase peptide synthesis (SPPS). It is functionalized with Fmoc-protected L-phenylalanine (Fmoc-Phe-OH) via an ester linkage to a Wang linker, anchored on a 100–200 mesh polystyrene-divinylbenzene (PS-DVB) matrix. Upon cleavage with trifluoroacetic acid (TFA), peptides synthesized on this resin bear a C-terminal free carboxylic acid (-COOH).
This resin is an ideal starting point for synthesizing C-terminal phenylalanine peptides, saving time by eliminating the first amino acid coupling step.
Specifications
Matrix: Polystyrene cross-linked with ~1% DVB
Linker: Wang (acid-labile ester linkage)
Preloaded Amino Acid: Fmoc-L-Phenylalanine (Fmoc-Phe-OH)
Functional Group: Esterified phenylalanine (–COO–Resin)
Loading: Typically 0.6–1.1 mmol/g (varies by manufacturer)
Particle Size: 100–200 mesh (approx. 75–150 µm)
Compatible Chemistry: Fmoc-based SPPS
Final Product: Peptide with C-terminal –COOH
Key Features
Preloaded with Fmoc-Phe: Ready-to-use for efficient SPPS
C-Terminal Acid: Ideal for biologically active peptides and pharmacological studies
Standard Mesh Size: Compatible with most synthesizers and manual protocols
Fmoc-Compatible: Fully compatible with standard Fmoc/tBu workflows
Stable & Reliable: Acid-labile Wang linker offers clean cleavage under TFA
Applications
Peptide synthesis with C-terminal phenylalanine
Biologically active peptides or hormone analogs
Pharmacological studies and SAR exploration
Library synthesis for screening or drug discovery
Peptides requiring C-terminal free acid functionality
Cleavage & Deprotection
Cleavage Cocktail: 95% TFA + 2.5% water + 2.5% TIS (or equivalent)
Cleavage Time: 1–3 hours at room temperature
Result: Peptide with a C-terminal –COOH, fully deprotected
Handling & Storage
Store dry and protected from light and moisture
Swell in DCM or DMF before use
Compatible with manual or automated peptide synthesizers
Stable under standard SPPS conditions (base washes, acid deprotection)
Comparison With Similar Resins
| Property | Fmoc-Phe-Wang Resin | Fmoc-Phe-Rink Amide Resin | Fmoc-Phe-2-Cl-Trityl Resin |
|---|---|---|---|
| C-Terminal Group | –COOH (acid) | –CONH₂ (amide) | –COOH (acid, milder cleavage) |
| Cleavage Conditions | TFA (95%) | TFA (95%) | Mild acid (1–3% TFA) |
| Stability | High | High | Moderate |
Conclusion
Fmoc-Phe-Wang Resin (100–200 mesh) offers a convenient and efficient solution for the synthesis of peptides with C-terminal phenylalanine and free carboxylic acid termini. Its ready-to-use format saves time while providing high loading capacity, excellent compatibility with standard SPPS protocols, and high-quality peptide products.
Phone : +49 1512 4714765 ( Germany )
Phone : +1 (702) 381-3042 ( USA )
Email : [email protected]
Address : Cunostraße 56, 14193 Berlin. Germany
Address : 7373 Rowlett Park Dr, Tampa, FL 33610, United States.